Step-by-Step Guide #

So, you’ve some histological images and a good dose of scientific curiosity. Now what? Let’s walk through the pipeline together, breaking it down step by step.

1. Prepare Your Data
   • 1.1 Stain the histological images
   • 1.2 Ensure the images are formatted correctly
   • 1.3 Remove image artifacts
2. Segment Vessels and Extract Features in Python
   • 2.1 Convertion of RGB to HSV format
   • 2.2 Application of Otsu’s thresholding
   • 2.3 Morphological operations
   • 2.4 Feature extraction
3. Statistical Analyses
   • 3.1 Normalization
4. Infer Drug Distribution
   • 4.1 Generation of synthetic vessel masks
   • 4.2 Definition of a known drug distribution function
   • 4.3 Application of GP modelling on simulator
   • 4.4 Assesment of model accuracy
5. Interpret Your Data